Effects of different sterilents on seeds and Callusing frequency as effected by hormones in Nicotiana tabacum L.
Author: Syed Abdul Azeem
Author affiliation: Department of Biotechnology, Sarhad University of Science and Information Technology, Peshawar, Pakistan
Email: bdlazeem@gmail.com
Supervisors: Ihsan Ullah1, Muhammad Ali1, Akram Khan 1, Jehan Bakht2
Supervisors affiliation: 1 Department of Biotechnology, Sarhad University of Science and Information Technology, Peshawar, Pakistan.
2Institute of biotechnology and Genetic Engineering NWFP, Agricultural University Peshawar, Pakistan
Dates: Received on: 11 February 2010, Accepted on: 20 February 2010, Published on 2 April 2010
Article type: Biotechnology
Abstract
In our study an attempt has been made to analyze the effects of different sterilents on the survival rate of tobacco seeds (Nicotiana tabacum L) and the role of different growth hormones on callogenesis. The seeds of tobacco were surface disinfected with two different types of sterilents, Calcium Hypo Chloride (CaOCl2) (2.0% and 4.0%) and Mercuric Chloride (HgCl2) (0.2% and 0.05%) for a particular time interval. The maximum survival percentages (93.33%) of seeds obtained at 0.2% Mercuric Chloride (HgCl2). While in the second half of the study, frequencies of callus formation under the influence of various growth hormones were analyzed in tobacco. In this regard, the Murashige and Skoog (MS) media were supplemented with different hormones; glutamine at 200 mgl-1 and 250 mgl-1, 6-Benzyle amino purine (BAP) at 1mgl-1 and 2,4-Dichlorophenoxy acetic acid (2,4D) at 2 mgl-1 and 3 mgl-1 respectively. Frequency of callus formation was observed and compared with control treatment. The maximum (81.7%) callusing was observed using 2, 4D at 3 mgl-1, with vigorous and green spots
Citation
Syed Abdul Azeem, Ihsan Ullah, Muhammad Ali, Akram Khan, Jehan Bakht, Effects of different sterilents on seeds and Callusing frequency as effected by hormones in Nicotiana tabacum L. Biofrontiers (2010) vol.1, Issue.2: Pg 62-67
| Publisher | Biofrontier Publications |
| ISSN | 0975-4865 |
| Copyright | Author |
| License | Creative Commons Attribution-Noncommercial 3.0 Unported License |
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